Part:BBa_R0080:Experience

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User Reviews

UNIQe1f155d666c3759b-partinfo-00000000-QINU

•• Xinyuan Qiu NUDT_CHINA2014

This promoter is lack of AraC gene so that the promoter can only activate the down-stream expression with the existence of both AraC and Arabinose Materials and Methods We used the circuit of BBa_K1532008 to test the function of this part, the sequence and features of the part BBa_K1532008 is as follows: Fluorometric measurement were processed with Fluoroskan(R) Ascent FL from ThermoTM using the filters of 485nm and 538nm. The E.coli to be tested was cultured in liquid LB media containing 35μg/mL chloramphenicol to OD1.6, than split into 96 well plates (from ThermoTM) by 150μL/well. The plate was then put into the Fluoroskan(R) Ascent FL and cultured in 25℃ and background shake of 90rpms. The Fluorometric measurement was processed with the interval time of 10 minutes. The layout of the plate is as follows: We draw the curve using the intensity figure of different amount of Arabinose subtracted by the figures of those without Arabinose. Results The result is as follows: The results indicates that with the existence of Arabinose and AraC, the part BBa_R0080 can activate the down-stream expression observably.

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Stephanie

The promoter is not inducible by arabinose as it does not contain the required araC gene. Use I0500 instead which contains the araC gene and the Pbad arabinose controlled promoter.

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iGEM TUDelft 2008, Ostassen

We've used this promoter in our project, and have also found it is always on, independent of arabinose. This result can be found [http://2008.igem.org/Team:TUDelft/Temperature_results#Setting_up_luciferase_measurements here] in figure 3, and has been confirmed on more occasions later.

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